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Immunocytochemical Localization of Prostaglandin EP3 Receptor in the Rat Hypothalamus

K Nakamura, T Kaneko, Y Yamashita, H Hasegawa, H Katoh, A Ichikawa, M Negishi

Neurosci Lett. 1999 Jan 29;260(2):117-20.

PMID: 10025713

Abstract:

A rabbit antibody against an N-terminal portion of rat prostaglandin EP3 receptor (EP3R) was produced to examine the distribution of EP3R in the rat hypothalamus. The antibody specifically recognized EP3R proteins in rat brain extract, in membrane fractions of rat kidney, and in membrane fractions of EP3R-expressing culture cells. Intense EP3R-like immunoreactivity was observed in the median preoptic nucleus, medial preoptic area, parastrial nucleus, compact part of the dorsomedial hypothalamic nucleus, and dorsal part of the premammillary nucleus. These results suggest that prostaglandin E2 mediates various actions in the hypothalamus, such as fever induction in the preoptic area, through EP3R.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
IAR4245810 Anti-hnRNP-E1/E2 (N-terminal) antibody produced in rabbit Anti-hnRNP-E1/E2 (N-terminal) antibody produced in rabbit Price
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