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Immunohistochemical Assessment of Hepatitis C Virus Antigen in Cholestatic Hepatitis After Liver Transplantation

Immunohistochemical Assessment of Hepatitis C Virus Antigen in Cholestatic Hepatitis After Liver Transplantation

F Fenwick, M F Bassendine, K Agarwal, D Bevitt, W Pumeechockchai, A D Burt, G L Toms

J Clin Pathol. 2006 Feb;59(2):174-8.

PMID: 16443734

Abstract:

Background:
Patients with common variable immunodeficiency may exhibit rapidly progressive hepatitis when infected with hepatitis C virus (HCV), leading to cirrhosis and liver failure. Liver transplantation in these patients may result in a cholestatic form of HCV reinfection with exceptionally high virus loads.
Aims:
To report an immunohistochemical investigation of the pretransplant and post-transplant liver of one such patient.
Methods/results:
On immunohistochemical staining of frozen sections with anti-HCV core monoclonal antibody or fluorescein labelled human polyclonal anti-HCV IgG, no HCV antigens were demonstrated in the native cirrhotic liver removed at transplant, despite a viral load of 10(6.4) genomes/g. The transplanted liver, collected six weeks post-transplant, exhibited cholestatic recurrent hepatitis, had an HCV virus load of 10(10) genomes/g of liver, and revealed HCV antigen in the cytoplasm of most hepatocytes, with a pronounced periportal distribution. No virus antigen was demonstrable in other cell types. The core antigen was also detected in paraffin wax embedded, formaldehyde fixed tissue of this liver after high temperature antigen retrieval, but not in the native cirrhotic liver or a selection of HCV positive livers collected pretransplant from immunocompetent patients. Attempts to delineate the distribution of E1, NS3, and NS4 antigens were unsuccessful because monoclonal antibodies to these antigens produced "false positive" staining of foci of hepatocytes in the post-transplant livers of HCV seronegative patients with cholestasis.
Conclusion:
This case provided an opportunity to study the natural development of HCV during acute infection in the absence of an immune response, and may help to elucidate the pathogenesis of HCV recurrence in liver allografts.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42414625	Hepatitis C virus core antigen−Fluorescein			Price
IAR42414633	Hepatitis C virus NS4 antigen−Fluorescein			Price

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