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Immunolocalization of the Toxin Latrunculin B Within the Red Sea Sponge Negombata Magnifica (Demospongiae, Latrunculiidae)

Immunolocalization of the Toxin Latrunculin B Within the Red Sea Sponge Negombata Magnifica (Demospongiae, Latrunculiidae)

O Gillor, S Carmeli, Y Rahamim, Z Fishelson, M Ilan

Mar Biotechnol (NY). 2000 May;2(3):213-23.

PMID: 10852799

Abstract:

The location of latrunculin B, the major toxin of the Red Sea sponge Negombata magnifica, was revealed using specific antibodies. Antibodies from rabbits immunized with a conjugate of latrunculin B with keyhole limpet hemocyanin (KLH) were purified over a latrunculin B-Sepharose affinity column. Analysis of immunohistochemical and immunogold-stained sponge sections, using light and transmission electron microscopy, revealed latrunculin B labeling mostly beneath the sponge cortex at the border between the external (ectosome) and internal (endosome) layers (ectosome-endosome border). The endosome was less labeled than the border. Immunogold localization revealed latrunculin B in the sponge cells but not in its prokaryotic symbionts. Archeocytes and choanocytes were significantly more labeled than other cells. The antibodies primarily labeled membrane-limited vacuoles within archeocytes and choanocytes that are perhaps latrunculin B secretory or storage vesicles. Peripheral latrunculin B may have a role in defense against external epibionts, predators, and competitors.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP76343947	Latrunculin B from Latruncula magnifica		76343-94-7	Price

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