0

Improved Conditions for periodate/Schiff's Base-Based Fluorescent Staining of Glycoproteins With Dansylhydrazine in SDS-PAGE

Xuan Zhou, Guo-Ying Hong, Bin-Bin Huang, Yuan-Meng Duan, Jia-Yi Shen, Mao-Wei Ni, Wei-Tao Cong, Li-Tai Jin

Electrophoresis. 2014 May;35(10):1439-47.

PMID: 24591039

Abstract:

An improved periodate/Schiff's base based fluorescent stain with dansylhydrazine (DH) for glycoproteins in 1D and 2D SDS-PAGE was described. Down to 4-8 ng of glycoproteins can be selectively detected within 2 h, which is approximately 16-fold higher than that of original protocol, but similar to that of Pro-Q Emerald 488 stain (Invitrogen, Carlsbad, USA). Furthermore, subsequent study of deglycosylation, glycoprotein affinity isolation, and LC-MS/MS analysis were performed to confirm the specificity of the improved method. As a result, improved DH stain may provide a new choice for selective, economic, MS compatible, and convenient visualization of gel-separated glycoproteins.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP33008069 Dansylhydrazine Dansylhydrazine 33008-06-9 Price
qrcode