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Improved in Situ Beta-Galactosidase Staining for Histological Analysis of Transgenic Mice

Improved in Situ Beta-Galactosidase Staining for Histological Analysis of Transgenic Mice

A Aguzzi, F Theuring

Histochemistry. 1994 Dec;102(6):477-81.

PMID: 7535299

Abstract:

The present study describes a novel method for the histochemical demonstration of beta-galactosidase activity on tissue sections. We have replaced 5-bromo-4-chloro-3-indolyl-beta-D-galactoside (X-Gal) with 5-bromo-indolyl-beta-o-galactopyranoside (Bluo-Gal) as a chromogenic substrate for the bacterial beta-galactosidase (lacZ). After beta-galactosidic cleavage, Bluo-Gal precipitates in form of fine birefringent crystals, whereas X-gal gives rise to an amorphous precipitate. Upon microscopic examination under polarized light, the crystals emit a strong signal consisting of yellow reflected light. This property of Bluo-Gal results in greatly enhanced sensitivity of the staining method for beta-galactosidase and allows for optimal morphological resolution. To exemplify the applications of this technique, the expression is demonstrated in transgenic mice of beta-galactosidase driven by a fragment of the human tissue-type plasminogen activator promoter.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP97753827-B	Bluo-Gal		97753-82-7	Price

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