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Improved Rapid Detection of Trypsin Isoinhibitors Using Non-Denaturing Polyacrylamide Gels With Immobilised Azoalbumin

Improved Rapid Detection of Trypsin Isoinhibitors Using Non-Denaturing Polyacrylamide Gels With Immobilised Azoalbumin

Hossein Alizadeh, David W M Leung

Phytochem Anal. Jul-Aug 2011;22(4):374-7.

PMID: 21204154

Abstract:

Introduction:
Proteinaceous inhibitors of animal trypsin occur naturally as isoforms in seeds and some are of interest as antinutritional or anti-pest agents.
Objective:
To establish a simplified electrophorectic, in-gel method for rapid and direct detection of trypsin isoinhibitors present in crude plant extracts that are particularly suitable for many studies including rapid evaluation of cultivars.
Methodology:
Azoalbumin (3%, w/v) is immobilised in 7.5% polyacrylamide gels before electrophoresis under non-denaturing conditions.
Results:
This improved method eliminates the need for both time-consuming and labourious staining and destaining or renaturation steps.
Conclusion:
Immobilised azoalbumin in polyacrylamide gels, run under non-denaturing electrophoresis conditions, can be used to assist rapid evaluation of trypsin isoinhibitors in numerous crude plant extracts.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP102110736	Azoalbumin		102110-73-6	Price

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