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Improvement of Cellulase Production in Trichoderma Reesei Rut-C30 by Overexpression of a Novel Regulatory Gene Trvib-1

Improvement of Cellulase Production in Trichoderma Reesei Rut-C30 by Overexpression of a Novel Regulatory Gene Trvib-1

Fei Zhang, Xinqing Zhao, Fengwu Bai

Bioresour Technol. 2018 Jan;247:676-683.

PMID: 30060399

Abstract:

Trichoderma reesei is a widely used cellulase producer, and development of robust strains for improved cellulase production is of great interest. In this study, the gene Trvib-1 encoding a putative transcription factor was overexpressed in T. reesei Rut-C30, and effects on cellulase production by the manipulation as well as corn stover degradation by the crude enzyme were investigated. Cellulase production and protein secretion were significantly improved in the culture of the recombinant T. reesei Vib-1, which were 200% and 219%, respectively, higher than that produced by the parent strain. Cellulase induction was enhanced in the presence of pure cellulose as well as various soluble inducers. Glucose released from the pretreated corn stover hydrolyzed by the crude enzyme in the recombinant strain was improved 40%. These results indicate that the overexpression of Trvib-1 is a feasible strategy for producing cellulase to enhance bioconversion efficiency of lignocellulosic biomass.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP9012548-C	Cellulase from Trichoderma reesei		9012-54-8	Price

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