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Improvement of (R)-3-hydroxybutyric Acid Secretion During Halomonas Sp. KM-1 Cultivation With Saccharified Japanese Cedar by the Addition of Urea

Improvement of (R)-3-hydroxybutyric Acid Secretion During Halomonas Sp. KM-1 Cultivation With Saccharified Japanese Cedar by the Addition of Urea

Y Kawata, M Nojiri, I Matsushita, J Tsubota

Lett Appl Microbiol. 2015 Oct;61(4):397-402.

PMID: 26249654

Abstract:

Japanese cedar (Cryptomeria japonica) is a major species in artificial Japanese forests. The Halomonas sp. KM-1 was recently isolated and found to grow effectively on saccharified Japanese cedar wood, resulting in the intracellular storage of poly-(R)-3-hydroxybutyric acid (PHB) under aerobic conditions. Under microaerobic conditions, the extracellular secretion of (R)-3-hydroxybutyric acid ((R)-3-HB) led to the degradation of intracellular PHB. In this study, the production of PHB and the secretion of (R)-3-HB using saccharified Japanese cedar were much improved in cultures that were grown in the presence of urea. The level of intracellular PHB production after 36 h under aerobic cultivation was 23·6 g l(-1) ; after shifting to microaerobic conditions for 24 h, the (R)-3-HB concentration in the medium reached 21·1 g l(-1) . Thus, KM-1 efficiently utilizes saccharified Japanese cedar to produce PHB and secretes (R)-3-HB, making it a practical candidate for use in the industrial production of (R)-3-HB.
Significance and impact of the study:
Japanese cedar is a major species grown in artificial Japanese forests, and its thinning is crucial for the health of artificial forests and the Japanese economy. Halomonas sp. KM-1 grew effectively on saccharified Japanese cedar wood, resulting in intracellular storage of poly-(R)-3-hydroxybutyric acid (PHB) under aerobic conditions. Under microaerobic conditions, extracellular secretion of (R)-3-hydroxybutyric acid ((R)-3-HB) caused intracellular PHB degradation. (R)-3-HB is a chiral compound that is useful in the chemical, health food and pharmaceutical industries. The production of PHB and secretion of (R)-3-HB using saccharified wood was dramatically improved, which may positively affect its future industrial production.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP29435481	Poly[(R)-3-hydroxybutyric acid]		29435-48-1	Price
AP625729	(R)-3-Hydroxybutyric acid		625-72-9	Price

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