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Improvement of the Production Efficiency of L-(+)-tartaric Acid by Heterogeneous Whole-Cell Bioconversion

Improvement of the Production Efficiency of L-(+)-tartaric Acid by Heterogeneous Whole-Cell Bioconversion

Ziqiang Wang, Yunshan Wang, Hong Shi, Zhiguo Su

Appl Biochem Biotechnol. 2014 Apr;172(8):3989-4001.

PMID: 24599671

Abstract:

An Escherichia coli-engineered bacterium with cis-epoxysuccinate hydrolase (ESH) activity was used to catalyze the stereospecific hydrolysis of cis-epoxysuccinic acid to L-(+)-tartaric acid. The effect of the substrate composition on the production efficiency of L-(+)-tartaric acid was investigated. Based on the sodium-type homogeneous substrate system, a heterogeneous substrate system, composed of 1.2 M sodium-type substrate and 1.8 M calcium-type substrate, was designed to improve ESH catalytic efficiency. After process optimization, a catalytic efficiency of 9.37 × 10(-3) g U(-1) h(-1) was obtained with fed-batch mode in the heterogeneous substrate system, about a twofold increase compared to the traditional bioconversion process with Nocardia tartaricans cells. The scale-up tests were carried out in a 15-m(3) stirred tank reactor, which indicated that the heterogeneous substrate system had great application prospect for the L-(+)-tartaric acid industrial production.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP87694-A	L-(+)-Tartaric acid		87-69-4	Price

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