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Improvements and Simplifications in In-Gel Fluorescent Detection of Proteins Using Ruthenium II Tris-(Bathophenanthroline Disulfonate): The Poor Man's Fluorescent Detection Method

Improvements and Simplifications in In-Gel Fluorescent Detection of Proteins Using Ruthenium II Tris-(Bathophenanthroline Disulfonate): The Poor Man's Fluorescent Detection Method

Catherine Aude-Garcia, Véronique Collin-Faure, Sylvie Luche, Thierry Rabilloud

Proteomics. 2011 Jan;11(2):324-8.

PMID: 21204259

Abstract:

Fluorescent detection of proteins is a popular method of detection allying sensitivity, linearity and compatibility with mass spectrometry. Among the numerous methods described in the literature, staining with ruthenium II tris(bathophenanthroline disulfonate) is particularly cost-effective, but slightly cumbersome owing to difficulties in the preparation of the complex and complexity of staining protocols. We describe here the modifications on both aspects that allow to perform a higher contrast staining and offer a more robust method of complex preparation, thereby maximizing the advantages of the method.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP1662017	Bathophenanthroline		1662-01-7	Price

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