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In Situ Assessment of Beta-Hexosaminidase Activity

In Situ Assessment of Beta-Hexosaminidase Activity

H D Lacorazza, M Jendoubi

Biotechniques. 1995 Sep;19(3):434-40.

PMID: 7495557

Abstract:

We have adapted two methods to evaluate the beta-hexosaminidase (HEX) enzymatic activity in cultured cells, based on the use of (i) the fluorogenic substrate 4-methylumbelliferyl-6-sulfo-2-acetamido-2- deoxy-beta-D-glucopyranoside (MU-GlcNAc-6-SO4) and (ii) the naphthol AS-BI-N-acetyl-beta-D-glucosaminide and hexazotized pararosaniline. We demonstrate that both methods could be used for the HEX isoenzymes by comparing wild-type and mutant human fibroblast cell lines, deficient for either an alpha or beta subunit from Tay-Sachs and Sandhoff patients. This in situ cytochemical assessment of HEX activity offers a rapid evaluation to study the expression of this enzyme in a heterogeneous cell population such as in gene transfer experiments.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP3395377	Naphthol AS-BI N-acetyl-β-D-glucosaminide		3395-37-7	Price

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