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In-situ Chemical Analyses of Trans-Polyisoprene by Histochemical Staining and Fourier Transform Infrared Microspectroscopy in a Rubber-Producing Plant, Eucommia Ulmoides Oliver

In-situ Chemical Analyses of Trans-Polyisoprene by Histochemical Staining and Fourier Transform Infrared Microspectroscopy in a Rubber-Producing Plant, Eucommia Ulmoides Oliver

Takeshi Bamba, Ei-Ichiro Fukusaki, Yoshihisa Nakazawa, Akio Kobayashi

Planta. 2002 Oct;215(6):934-9.

PMID: 12355153

Abstract:

The localization of polyisoprene in young stem tissues of Eucommia ulmoides Oliver was investigated by histochemical staining and Fourier transform infrared (FT-IR) microspectroscopy. The fibrous structures were stained with Oil Red O. FT-IR microspectroscopic analysis proved that the fibrous structures were trans-polyisoprene. Granular structures stained with the dye, and characteristic absorptions at 2,960 cm(-1) and 1,430 cm(-1) in FT-IR suggested that trans-polyisoprene accumulated in the vicinity of the cambium layer. We have thus successfully shown for the first time the localization of trans-polyisoprene in plant tissues, and our histological investigation allowed us to presume the main sites of biosynthesis and accumulation of trans-rubber. Furthermore, a new technical approach, the preparation of sections using an electronic freezing unit and the in situ analysis of polyisoprene using FT-IR microspectroscopy, is demonstrated to be a promising method for determining the accumulation of polyisoprene as well as other metabolites.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP104389324	Polyisoprene, trans		104389-32-4	Price

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