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In Situ Detection of the Zn(2+) Release Process of ZnO NPs in Tumour Cells by Confocal Laser Scanning Fluorescence Microscopy

In Situ Detection of the Zn(2+) Release Process of ZnO NPs in Tumour Cells by Confocal Laser Scanning Fluorescence Microscopy

Wenshuang Song, Xiaoling Tang, Yong Li, Yang Sun, Jilie Kong, Ren Qingguang

IET Nanobiotechnol. 2016 Aug;10(4):178-83.

PMID: 27463786

Abstract:

The use of zinc oxide (ZnO) nanoparticles (NPs) for cancer is not yet clear for human clinical applications, which is primarily due to the lack of a better understanding of the action mechanisms and cellular consequences of the direct exposure of cells to these NPs. In this work, the authors have selected zinquin ethyl ester, a Zn(2+)-specific fluorescent molecular probe, to efficiently differentiate ZnO NPs and Zn(2+), and combined with confocal laser scanning microscopy (CLSM) to in situ study the Zn(2+) release process of ZnO NPs in cancer cell system through detecting the change of Zn(2+) level over time. During the experiments, the authors have designed the test group ZnO-2 in addition to assess the influence of a long-term storage on the characteristics of ZnO NPs in aqueous solution, and the Zn(2+) release process of ZnO NPs in cancer cell system. After three-month storage at room temperature, the release process became earlier and faster, which was consistent with previous results of transmission electron microscope, UV-Vis and PL spectra. It is a good detection method that combination of Zn(2+)-specific fluorescent molecular probe and CLSM, which will be helpful for ZnO NPs using in clinical research.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42418017	Zinquin ethyl ester			Price

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