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In Situ Hybridization With Novel Biotinyl-Tyramide: Fundamental Studies and Its Utility of the Detection of Human Papilloma Virus in Tissue Sections

In Situ Hybridization With Novel Biotinyl-Tyramide: Fundamental Studies and Its Utility of the Detection of Human Papilloma Virus in Tissue Sections

Toshiaki Hikino

Rinsho Byori. 2007 Oct;55(10):922-9.

PMID: 18050669

Abstract:

We developed an in situ hybridization (ISH) method with a higher sensitivity and less background staining than the generally used catalyzed reporter deposition amplification method of in situ hybridization (CARD-ISH). The characteristics of this method are follows: sections heated in citrate buffer (pH6.0) containing 0.1% Triton X-100 showed the strongest signals, and a well-preserved morphology. The strongest signals were observed when borate buffer of biotinyl-tyramide stock and phosphate buffer of working solution were changed to Tris-HCl buffer. Compared with hematoxylin counter staining, alcian blue counter staining made it easier to identify dot, diffuse, and mixed type signals, respectively. Thus, we were able to clearly detect positive signals in the SiHa cell line with 1-2 copies of the integrated human papilloma virus-16 (HPV-16) gene, and in formalin-fixed, paraffin-embedded cervical lesion specimens for the HPV-16 gene.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP41994029	Biotinyl tyramide		41994-02-9	Price

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