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In Situ Polymerization of poly(γ-benzyl-l-glutamate) on Mesoporous Hydroxyapatite With High Graft Amounts for the Direct Fabrication of Biodegradable Cell Microcarriers and Their Osteogenic Induction

In Situ Polymerization of poly(γ-benzyl-l-glutamate) on Mesoporous Hydroxyapatite With High Graft Amounts for the Direct Fabrication of Biodegradable Cell Microcarriers and Their Osteogenic Induction

Linlong Li, Xincui Shi, Zongliang Wang, Yu Wang, Zixue Jiao, Peibiao Zhang

J Mater Chem B. 2018 May 28;6(20):3315-3330.

PMID: 32254389

Abstract:

Large-scale cell culture for cell expansion in tissue engineering is currently a major focus of research. One method to achieve better cell amplification is to utilize microcarriers. In this study, different amounts of poly(γ-benzyl-l-glutamate) (PBLG) (from 11 wt% to 50 wt%) were grafted on mesoporous hydroxyapatite (MHA) by the in situ ring opening polymerization of γ-benzyl-l-glutamate N-carboxyanhydride (BLG-NCA), and biodegradable and biocompatible PBLG-g-MHA microcarriers were directly fabricated using the oil-in-water (O/W) solvent-evaporation technique for bone tissue engineering. The amount of grafted PBLG could be controlled by adjusting the feed ratio of MHA and BLG-NCA. The relationships between sphere morphology and graft amount or solution concentration were explored. Furthermore, cytological assays were performed to evaluate the biological properties of the PBLG-g-MHA microcarriers. For a solution concentration of 3% (w/v) and PBLG graft amounts of 33 wt% and 50 wt%, the microspheres could be harvested with optimal spherical shapes. In vitro cell culture revealed that the PBLG-g-MHA microspheres had favorable properties for cell proliferation and significantly enhanced the osteogenic differentiation of MC3T3-E1 cells and bone matrix formation.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP25014271	Poly-γ-benzyl-L-glutamate		25014-27-1	Price

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