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In-time and In-Space Tandem Mass Spectrometry to Determine the Metabolic Profiling of Flavonoids in a Typical Sweet Cherry (Prunus Avium L.) Cultivar From Southern Italy

In-time and In-Space Tandem Mass Spectrometry to Determine the Metabolic Profiling of Flavonoids in a Typical Sweet Cherry (Prunus Avium L.) Cultivar From Southern Italy

Pasquale Crupi, Rosalinda Genghi, Donato Antonacci

J Mass Spectrom. 2014 Oct;49(10):1025-34.

PMID: 25303392

Abstract:

This paper presents a comprehensive analytical methodology, based on 'in-time' and 'in-space' tandem mass spectrometry (MS) techniques, to identify and quantify flavonoid compounds in a typical Italian sweet cherry cultivar (cv. Ferrovia). Five anthocyanins, four flavan-3-ols and nine flavonols were determined by means of hyphenated high-performance liquid chromatography - multi-stage MS (HPLC-MS(n)) analyses (MS(n) up to MS(4)), among which quercetin-3-O-rutinoside-7-O-glucoside, kaempferol-3-O-rutinoside-7-O-glucoside, quercetin-3-O-galactosyl-rhamnoside and quercetin-3-O-coumaroylglucoside were tentatively identified in sweet cherries for the first time. Ultrafast HPLC and tandem MS (UHPLC-MS/MS) analyses through multiple reaction monitoring experiments showed that cyanidin-3-O-rutinoside and cyanidin-3-O-glucoside were the main anthocyanins of cv. Ferrovia at maturity. Moreover, consistent levels of catechin and epicatechin as well as quercetin-3-O-rutinoside and kaempferol-3-O-rutinoside were also found. Because flavonoids have been ascribed as potential health-promoting compounds, gathered findings provide new insight into the knowledge of the quali-quantitative profile of these phytochemicals into a widespread fruit such as sweet cherry.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP30311616	Quercetin 3-O-rutinoside-7-O-glucoside		30311-61-6	Price

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