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In Vitro Biosynthesis of Juvenile Hormone in Larval Honey Bees: Comparison of Six Media

In Vitro Biosynthesis of Juvenile Hormone in Larval Honey Bees: Comparison of Six Media

A Rachinsky, K Hartfelder

In Vitro Cell Dev Biol Anim. 1998 Sep;34(8):646-8.

PMID: 9769150

Abstract:

We have formulated a tissue culture medium based on the components of larval honey bee hemolymph. Using an in vitro radiochemical assay to measure juvenile hormone biosynthesis, we compared our larval-based medium to four commercially available media (Grace's, Medium-199; Shields and Sang M3, and Minimum Essential Medium), and a medium based on adult honey bee hemolymph. All media were formulated without methionine. There was no significant difference in the amounts of juvenile hormone produced by the larval medium and Grace's; both of these media, however, were more suitable than the remaining four. Our larval-based tissue culture medium should prove useful in studies aimed at elucidating the underlying hormonal mechanism(s) of caste development in honey bees.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR4248443	Shields and Sang M3 Insect Medium			Price

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