Home
Resources
Publications
In Vitro Treatment With Concentrated Growth Factors (CGF) and Sodium Orthosilicate Positively Affects Cell Renewal in Three Different Human Cell Lines

In Vitro Treatment With Concentrated Growth Factors (CGF) and Sodium Orthosilicate Positively Affects Cell Renewal in Three Different Human Cell Lines

Veronica Bonazza, Elisa Borsani, Barbara Buffoli, Silvia Parolini, Francesco Inchingolo, Rita Rezzani, Luigi Fabrizio Rodella

Cell Biol Int. 2018 Mar;42(3):353-364.

PMID: 29105212

Abstract:

The aim of this study was to investigate the in vitro effect of Silicon, in the soluble form of sodium orthosilicate, combined and not with the concentrated growth factors (CGF), a platelet-rich preparation, on three different human cell lines of fibroblasts (NHDF), endothelial cells (HUVEC), and osteoblasts (HOBs). Each cell type was treated with sodium orthosilicate at the final concentration of 0.5 mM and 1 mM, CGF, and sodium orthosilicate combined with CGF, for 72 h. At the end of the experimental period, the in vitro effect on cell growth, proliferation, and metabolic activity was evaluated by performing a simple cell count, using an automated cell counter and by evaluating the expression of the intracellular proliferation marker Ki-67, using Fluorescence-activated cell sorting (FACS) analysis. Moreover, the expression of other cell markers and active molecules, such as Collagen type I, Osteopontin, Vascular Endothelial Growth Factor, and endothelial Nitric Oxide Synthase, was evaluated, through immunohistochemistry. Results obtained showed that the combined use of CGF and sodium orthosilicate stimulates cell growth, proliferation, and metabolic activity, suggesting that this treatment could be effective in tissue regeneration.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP80396709	Tetramethyl-d12 orthosilicate		80396-70-9	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: