Home
Resources
Publications
In Vivo Measurement of Tissue Oxygenation by Time-Resolved Luminescence Spectroscopy: Advantageous Properties of dichlorotris(1, 10-phenanthroline)-ruthenium(II) Hydrate

In Vivo Measurement of Tissue Oxygenation by Time-Resolved Luminescence Spectroscopy: Advantageous Properties of dichlorotris(1, 10-phenanthroline)-ruthenium(II) Hydrate

Veronika Huntosova, Sandrine Gay, Patrycja Nowak-Sliwinska, Senthil Kumar Rajendran, Matthieu Zellweger, Hubert van den Bergh, Georges Wagnières

J Biomed Opt. 2014;19(7):77004.

PMID: 25036215

Abstract:

Measuring tissue oxygenation in vivo is of interest in fundamental biological as well as medical applications. One minimally invasive approach to assess the oxygen partial pressure in tissue (pO2) is to measure the oxygen-dependent luminescence lifetime of molecular probes. The relation between tissue pO2 and the probes' luminescence lifetime is governed by the Stern-Volmer equation. Unfortunately, virtually all oxygen-sensitive probes based on this principle induce some degree of phototoxicity. For that reason, we studied the oxygen sensitivity and phototoxicity of dichlorotris(1, 10-phenanthroline)-ruthenium(II) hydrate [Ru(Phen)] using a dedicated optical fiber-based, time-resolved spectrometer in the chicken embryo chorioallantoic membrane. We demonstrated that, after intravenous injection, Ru(Phen)'s luminescence lifetime presents an easily detectable pO2 dependence at a low drug dose (1 mg∕kg) and low fluence (120 mJ∕cm2 at 470 nm). The phototoxic threshold was found to be at 10 J∕cm2 with the same wavelength and drug dose, i.e., about two orders of magnitude larger than the fluence necessary to perform a pO2 measurement. Finally, an illustrative application of this pO2 measurement approach in a hypoxic tumor environment is presented.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP207802457	Dichlorotris(1,10-phenanthroline)ruthenium(II) hydrate		207802-45-7	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: