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In Vivo Microsampling to Capture the Elusive Exposome

In Vivo Microsampling to Capture the Elusive Exposome

Vincent Bessonneau, Jennifer Ings, Mark McMaster, Richard Smith, Leslie Bragg, Mark Servos, Janusz Pawliszyn

Sci Rep. 2017 Mar 7;7:44038.

PMID: 28266605

Abstract:

Loss and/or degradation of small molecules during sampling, sample transportation and storage can adversely impact biological interpretation of metabolomics data. In this study, we performed in vivo sampling using solid-phase microextraction (SPME) in combination with non-targeted liquid chromatography and high-resolution tandem mass spectrometry (LC-MS/MS) to capture the fish tissue exposome using molecular networking analysis, and the results were contrasted with molecular differences obtained with ex vivo SPME sampling. Based on 494 MS/MS spectra comparisons, we demonstrated that in vivo SPME sampling provided better extraction and stabilization of highly reactive molecules, such as 1-oleoyl-sn-glycero-3-phosphocholine and 1-palmitoleoyl-glycero-3-phosphocholine, from fish tissue samples. This sampling approach, that minimizes sample handling and preparation, offers the opportunity to perform longitudinal monitoring of the exposome in biological systems and improve the reliability of exposure-measurement in exposome-wide association studies.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP19420565	1-Oleoyl-sn-glycero-3-phosphocholine		19420-56-5	Price

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