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In Vivo Optical Imaging of Membrane-Type Matrix Metalloproteinase (MT-MMP) Activity

In Vivo Optical Imaging of Membrane-Type Matrix Metalloproteinase (MT-MMP) Activity

Lei Zhu, Fan Zhang, Ying Ma, Gang Liu, Kwangmeyung Kim, Xuexun Fang, Seulki Lee, Xiaoyuan Chen

Mol Pharm. 2011 Dec 5;8(6):2331-8.

PMID: 22014151

Abstract:

Herein we demonstrate for the first time that a fluorogenic probe can be used as an in vivo imaging agent for visualizing activities of membrane-tethered, membrane-type matrix metalloproteinases (MT-MMPs). An MT-MMP fluorogenic probe that consisted of an MT1-MMP (MMP-14) substrate and near-infrared (NIR) dye-quencher pair exhibited rapid, efficient boosts in fluorescence upon cleavage by MT1-MMP in tumor-bearing mice. In particular, unlike similar fluorogenic probes designed to target extracellular, soluble-type MMPs (EC-MMPs)--which can be cleared from the bloodstream after activation--the fluorescence signals activated by MT1-MMP enable clear visualization of MT1-MMP-positive tumors in animal models for up to 24 h. The results indicate that a simple form of a fluorogenic probe that is less effective in EC-MMP imaging is an effective probe for imaging MT-MMP activities in vivo. These findings can be widely applied to designing probes and to applications targeting various membrane-anchored proteases in vivo.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42416011	MMP-14 Substrate I, Fluorogenic			Price

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