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In Vivo Visualization of Microglia Using Tomato Lectin

Bianca Brawek, Maria Olmedillas Del Moral, Olga Garaschuk

Methods Mol Biol. 2019;2034:165-175.

PMID: 31392684

Abstract:

Plant lectins bind to carbohydrates, which are found on the surface of different immune and endothelial cells including microglia. Using commercially available conjugates of lectins with different fluorophores allows one-step detection and visualization of microglia in vivo. Here, we describe a protocol enabling the use of a specific plant lectin isolated from Lycopersicon esculentum. Tomato lectin enables high-quality labeling of microglial cells in vivo and is applicable in any mouse strain at any age of the experimental animal without the need of genetic labeling, which is associated with time- and resource-consuming procedures.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
IAR424654 Lectin from Lycopersicon esculentum (tomato) Lectin from Lycopersicon esculentum (tomato) Price
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