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Increased Levels of Cytosolic Thioredoxin Reductase Activity and mRNA in Rat Liver Nodules

Increased Levels of Cytosolic Thioredoxin Reductase Activity and mRNA in Rat Liver Nodules

L Björkhem, H Teclebrhan, E Kesen, J M Olsson, L C Eriksson, M Björnstedt

J Hepatol. 2001 Aug;35(2):259-64.

PMID: 11580149

Abstract:

Background/aims:
Thioredoxin reductase, a redox active enzyme, is induced in several tumors. This study focuses on the presence of and subcellular localisation of thioredoxin reductase in a tumor model where neoplastic lesions are selected by their resistance to the toxic effects of the promotor.
Methods:
Liver nodules produced by intermittent feeding of 2-acetylaminofluorene to male Wistar rats were analyzed for thioredoxin reductase (TrxR) activity and mRNA.
Results:
This activity was increased 3.5-fold in the cytosol but decreased 60% in the mitochondrial fraction compared to the liver of age-matched untreated animals. Only traces of activity were observed in the microsomal, plasma membrane and nuclear fractions from normal liver or nodules. The level of TrxR mRNA was 3-fold higher in nodules than in normal rat liver. Furthermore, the total level of SH groups in homogenates was 2-fold higher in the case of the nodules.
Conclusions:
These findings indicate that the thioredoxin system makes an important contribution to the resistant phenotype of the neoplastic liver cell, which conveys a growth advantage of significance for tumor progression.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP9074140	Thioredoxin Reductase from rat liver		9074-14-0	Price

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