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Induction of Apoptosis by 2,3-dehydrosilybin via a Caspase-Dependent Pathway in Human HeLa Cells

Induction of Apoptosis by 2,3-dehydrosilybin via a Caspase-Dependent Pathway in Human HeLa Cells

Byoung Ok Cho, Yangkang So, Chang Hyun Jin, Myung Woo Byun, Kwon Il Seo, Kisung Ko, Myoung Sook Chun, Il Yun Jeong

Biosci Biotechnol Biochem. 2014;78(2):255-62.

PMID: 25036678

Abstract:

The aim of this study was to investigate the mechanisms involved in the apoptosis of HeLa cells due to 2,3-dehydrosilybin (DHS) treatment. DHS treatment over 24 h significantly inhibited cell viability and induced apoptosis in a dose-dependent manner. It also triggered the cleavage of caspase-8, caspase-9, caspase-3, and PARP, and significantly increased caspase-3 activity in a dose-dependent manner. Moreover, it triggered the depolarization of the mitochondrial membrane potential (Δψm), the release of cytochrome c into the cytosol, the cleavage of Bid, and the downregulation of Bcl-2 in a dose-dependent manner. Furthermore, z-VAD-fmk (a pan-caspase inhibitor) and z-IETD-fmk (a specific caspase-8 inhibitor) abolished the DHS-induced activation of the caspase-8, -9, and -3, cleavage of PARP, the depolarization of Δψm, the release of cytochrome c, the cleavage of Bid, and the downregulation of Bcl-2. Taken together, these results suggest that DHS-induced apoptosis is mediated by a caspase-dependent pathway in human HeLa cells.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP25166147	2,3-Dehydrosilybin A		25166-14-7	Price

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