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Induction of Apoptosis by Recombinant Soluble Human TRAIL in Jurkat Cells

Induction of Apoptosis by Recombinant Soluble Human TRAIL in Jurkat Cells

Gen-Hong Yao, Li-Jun Ling, Jian-Feng Luan, Dong Ye, Pei-Yuan Zhu, Qian-Hong Lei

Biomed Environ Sci. 2007 Dec;20(6):470-7.

PMID: 18348405

Abstract:

Objective:
To investigate the therapeutic potential of tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL), a member of the TNF superfamily, and to analyze TRAIL-induced apoptosis in Jurkat cells.
Methods:
Expression of TRAIL receptors (DR4 and DR5) was detected by reverse transcriptase-polymerase chain reaction (RT-PCR). Cytotoxic effects were determined by colony formation assay and a cell counting kit. The effects of recombinant TRAIL on apoptosis of Jurkat cells were determined by DNA fragmentation (DNA ladder) and PI staining. Changes in mitochondrial membrane potential were detected with JC-1 fluorescence.
Results:
TRAIL inhibited the proliferation and induced internucleosomal DNA fragmentation (characteristic of apoptosis) and loss of mitochondrial membrane potential.
Conclusion:
Recombinant soluble TRAIL can be used as a therapy for cancer.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42412947	TRAIL, Soluble human			Price

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