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Induction of CCL8/MCP-2 by Mycobacteria Through the Activation of TLR2/PI3K/Akt Signaling Pathway

Induction of CCL8/MCP-2 by Mycobacteria Through the Activation of TLR2/PI3K/Akt Signaling Pathway

Haipeng Liu, Zhonghua Liu, Jianxia Chen, Ling Chen, Xin He, Ruijuan Zheng, Hong Yang, Peng Song, Dong Weng, Haili Hu, Lin Fan, Heping Xiao, Stefan H E Kaufmann, Joel Ernst, Baoxue Ge

PLoS One. 2013;8(2):e56815.

PMID: 23418602

Abstract:

Pleural tuberculosis (TB), together with lymphatic TB, constitutes more than half of all extrapulmonary cases. Pleural effusions (PEs) in TB are representative of lymphocytic PEs which are dominated by T cells. However, the mechanism underlying T lymphocytes homing and accumulation in PEs is still incompletely understood. Here we performed a comparative analysis of cytokine abundance in PEs from TB patients and non-TB patients by protein array analysis and observed that MCP-2/CCL8 is highly expressed in the TB-PEs as compared to peripheral blood. Meanwhile, we observed that CCR5, the primary receptor used by MCP-2/CCL8, is mostly expressed on pleural CD4(+) T lymphocytes. Furthermore, we found that infection with either Mycobacterium bovis Bacillus Calmette-Guérin (BCG) or Mycobacterium tuberculosis H37Rv induced production of MCP-2/CCL8 at both transcriptional and protein level in Raw264.7 and THP-1 macrophage cells, mouse peritoneal macrophages as well as human PBMC monocyte-derived macrophages (MDMs). The induction of MCP-2/CCL8 by mycobacteria is dependent on the activation of TLR2/PI3K/Akt and p38 signaling pathway. We conclude that accumulation of MCP-2/CCL8 in TB-PEs may function as a biomarker for TB diagnosis.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42413136	MCP-2 from mouse			Price

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