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Influence of Hydrodynamic Conditions on Quantitative Cellular Assays in Microfluidic Systems

Huabing Yin, Xunli Zhang, Nicola Pattrick, Norbert Klauke, Hayley C Cordingley, Stephen J Haswell, Jonathan M Cooper

Anal Chem. 2007 Sep 15;79(18):7139-44.

PMID: 17658886

Abstract:

This study demonstrates the importance of the hydrodynamic environment in microfluidic systems in quantitative cellular assays using live cells. Commonly applied flow conditions used in microfluidics were evaluated using the quantitative intracellular Ca2+ analysis of Chinese hamster ovary (CHO) cells as a model system. Above certain thresholds of shear stress, hydrodynamically induced intracellular Ca2+ fluxes were observed which mimic the responses induced by chemical stimuli, such as the agonist uridine 5'-triphosphate tris salt (UTP). This effect is of significance given the increasing application of microfluidic devices in high-throughput cellular analysis for biophysical applications and pharmacological screening.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP108321535 Uridine 5′-triphosphate tris salt Uridine 5′-triphosphate tris salt 108321-53-5 Price
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