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Influence of Polypeptide Molecular and Side Chain Lengths and of Side Chain Steric Location on the Kinetics of Basic Polypeptide-Induced Sensitization of Primate Cells to Transfection

Influence of Polypeptide Molecular and Side Chain Lengths and of Side Chain Steric Location on the Kinetics of Basic Polypeptide-Induced Sensitization of Primate Cells to Transfection

G R Dubes, R J Wegrzyn

Acta Virol. 1977 Jan;21(1):15-24.

PMID: 15433

Abstract:

Kinetics of sensitization of chimpanzee cell sheets to transfection by poliovirus RNA was determined for 5 basic polypeptides. With basic olypeptide hydrobromide at 100 microng/ml, initial sensitization rate was faster for poly-L-ornithine of average molecular weight (AMW) 15500 than of AMW 105000, and much faster for poly-L-lysine of AMW 1700 than of AMW 140000. Desensitization phases were observed with the 2 shorter polypeptides. Sensitization was much faster and sensitivity maxima were considerably higher for the polyornithines than for the polylysines. Poly-D-lysine of AMW 160000 sensitized cells slightly faster than poly-L-lysine of AMW 140000, but gave about the same sensitivity maximum. Analysis of the slow cell sensitization by poly-L-lysine of AMW 140000 revealed 2 steps: a fast step 1 (attachment) and a slow step 2 (processing).

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP27378490-A	Poly-L-ornithine hydrobromide		27378-49-0	Price

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