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Influence of the Crosslinker Type on the Chromatographic Properties of Hydrophilic Sulfoalkylbetaine-Type Monolithic Columns

Influence of the Crosslinker Type on the Chromatographic Properties of Hydrophilic Sulfoalkylbetaine-Type Monolithic Columns

Chusheng Liu, Weijia Chen, Guangxin Yuan, Yao Xiao, Jacques Crommen, Shihai Xu, Zhengjin Jiang

J Chromatogr A. 2014 Dec 19;1373:73-80.

PMID: 25464999

Abstract:

In order to investigate the effects of the crosslinker on the separation performance of polar zwitterionic sulfoalkylbetaine-type monolithic columns, three crosslinkers, i.e. 1,4-bis(acryloyl)piperazine (PDA), ethylene dimethacrylate (EDMA) and N,N'-methylenebisacrylamide (MBA), were copolymerized with the hydrophilic monomer N,N-dimethyl-N-acryloyloxyethyl-N-(3-sulfopropyl)ammonium betaine (SPDA). The chromatographic properties of the three hydrophilic sulfoalkylbetaine-type monolithic columns, including column efficiency, permeability, porosity and separation mechanism, were systematically compared using scanning electron microscopy or micro-HPLC. Good selectivity in micro-HPLC separations was achieved on all three monolithic columns. The results indicate that the polarity of sulfoalkylbetaine-type monolithic columns may be related to the polarity of the crosslinker, which further affects column selectivity and efficiency. A particularly high column efficiency (100,000 plates/m) was obtained on the novel poly(SPDA-co-PDA) monolithic column at a linear velocity of 1mm/s using thiourea as test analyte. A higher resolution was also observed for nucleobases, nucleosides and hydrophilic organic acids on this novel poly(SPDA-co-PDA) monolithic column compared to the other two columns.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP6342172-A	1,4-Bis(acryloyl)piperazine		6342-17-2	Price

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