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Infrared-assisted Extraction Followed by High Performance Liquid Chromatography to Determine Angoroside C, Cinnamic Acid, and Harpagoside Content in Scrophularia Ningpoensis

Infrared-assisted Extraction Followed by High Performance Liquid Chromatography to Determine Angoroside C, Cinnamic Acid, and Harpagoside Content in Scrophularia Ningpoensis

Lina Su, Yinghui Deng, Nianzu Chen, Xiuwen Zhang, Taomin Huang

BMC Complement Altern Med. 2019 Jun 14;19(1):130.

PMID: 31200712

Abstract:

Background:
Angoroside C, cinnamic acid, and harpagoside are bioactive constituents in Scrophularia ningpoensis. Currently, an infrared-assisted extraction (IRAE) method coupled with high-performance liquid chromatography with ultraviolet detection (HPLC-UV) for the analysis of bioactive constituents in this plant is lacking.
Methods:
A method based on HPLC following IRAE has been developed for quantifying angoroside C, cinnamic acid, and harpagoside in Scrophularia ningpoensis. Four main factors, namely, extraction solvent, solid/liquid ratio, illumination time, and distance between the infrared lamp and the round-bottom flask, were optimized for extraction. Furthermore, conventional ultrasonic extraction (USE) and microwave-assisted extraction (MAE) were also investigated to validate the developed method.
Results:
The optimal extraction conditions were as follows: ethanol concentration, 37.5%; solid/liquid ratio, 1:25; illumination time, 10 min; and distance between infrared lamp and round-bottom flask, 3 cm. The results of method validation demonstrated that the developed method meets the requirement of analysis.
Conclusion:
The results show that the IRAE-HPLC is a simple, accurate, and green analytical preparatory method for the potential extraction and quantification of angoroside C, cinnamic acid, and harpagoside in Scrophularia ningpoensis.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP115909223	Angoroside C		115909-22-3	Price

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