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Inhibition of 2-aminoethoxydiphenyl Borate-Induced Rat Atrial Ectopic Activity by Anti-Arrhythmic Drugs

Inhibition of 2-aminoethoxydiphenyl Borate-Induced Rat Atrial Ectopic Activity by Anti-Arrhythmic Drugs

Rong Huo, Zhe Li, Cui-Cui Lu, Yan Xie, Bin Wang, Yu-Jie Tu, Jun-Tao Hu, Chang-Qing Xu, Bao-Feng Yang, De-Li Dong

Cell Physiol Biochem. 2010;25(4-5):425-32.

PMID: 20332623

Abstract:

Background/aims:
2-aminoethoxydiphenyl borate (2-APB) provokes spontaneous mechanical activity in isolated rat left atria. The present study is to characterize 2-APB-induced ectopic activity in rat atria and to investigate the inhibition of 2-APB-induced ectopic activity by anti-arrhythmic drugs.
Methods:
2-APB-induced ectopic activity was measured through an isometric force transducer connected to a multichannel acquisition and analysis system. Intracellular [Ca2+](i) was measured with fluorescence laser scanning confocal microscopy. Voltage-dependent L- type Ca2+ currents were recorded by using patch-clamp technique.
Results:
2-APB dose-dependently increased the ectopic activity of left atria at 1, 5, 10, 20, 50 microM. Anti-arrhythmic drugs, quinidine (10 microM), lidocaine (10 microM), verapamil (5 microM), and amiodarone (50 microM, 100 microuM) inhibited 2-APB-induced ectopic activity. 2-APB-induced ectopic activity was inhibited by Ca2+-free bath, Na+/Ca2+ exchanger blockers, 3',4'-dichlorobenzamil hydrochloride (DHC) and Ni2+, not by non-selective cation channel blocker Gd3+. 2-APB also induced ectopic contractions in ventricular tissue straps and the ectopic contractions were inhibited by quinidine, verapamil and DHC. Lidocaine, verapamil and DHC inhibited 2-APB-induced increase of intracellular Ca2+ concentration in cardiomyocytes. Low molecular weight heparin inhibited phenylephrine (PE)-induced but not 2-APB -induced atria ectopic activity, and the pattern of 2-APB-induced ectopic activity was continuous, distinct from the discontinuous activity induced by PE.
Conclusion:
2-APB-induced atria ectopic activity was inhibited by classic anti-arrhythmic drugs quinidine, lidocaine, verapamil, amiodarone, and Na+/Ca2+ exchanger blockers. It can be used for testing agents able to affect any of Na+, Ca2+ channel, Na+/Ca2+ exchanger without selectivity.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP9041081-I	Low Molecular Weight Heparin for Boron Analysis		9041-08-1	Price
AP928621152	3′,4′-Dichlorobenzamil hydrochloride		928621-15-2	Price

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