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Inhibition of Escherichia Coli Inorganic Pyrophosphatase by Fructose-1-phosphate

N N Vorobyeva, S A Kurilova, V A Anashkin, E V Rodina

Biochemistry (Mosc). 2017 Aug;82(8):953-956.

PMID: 28941463

Abstract:

Pyrophosphate regulates vital cellular reactions, and its level in E. coli cells is under the ultimate control of inorganic pyrophosphatase. The mechanisms involved in the regulation of pyrophosphatase activity still need to be elucidated. The present study demonstrated that fructose-1-phosphate inhibits pyrophosphatase activity by a mechanism not involving competition with substrate for binding to the active site. The inhibition constant governing the binding of the inhibitor to the enzyme-substrate complex is 1.1 mM. Substitutions of Lys112, Lys115, Lys148, and Arg43 in the regulatory site completely or partially abolished the inhibition. Thus, Fru-1-P is a physiological inhibitor of pyrophosphatase that acts via a regulatory site in this enzyme.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP9024822 Pyrophosphatase, Inorganic from Escherichia coli Pyrophosphatase, Inorganic from Escherichia coli 9024-82-2 Price
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