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Inhibition Studies of Soybean (Glycine Max) Urease With Heavy Metals, Sodium Salts of Mineral Acids, Boric Acid, and Boronic Acids

Inhibition Studies of Soybean (Glycine Max) Urease With Heavy Metals, Sodium Salts of Mineral Acids, Boric Acid, and Boronic Acids

Sandeep Kumar, Arvind M Kayastha

J Enzyme Inhib Med Chem. 2010 Oct;25(5):646-52.

PMID: 20014894

Abstract:

Various inhibitors were tested for their inhibitory effects on soybean urease. The K(i) values for boric acid, 4-bromophenylboronic acid, butylboronic acid, and phenylboronic acid were 0.20 +/- 0.05 mM, 0.22 +/- 0.04 mM, 1.50 +/- 0.10 mM, and 2.00 +/- 0.11 mM, respectively. The inhibition was competitive type with boric acid and boronic acids. Heavy metal ions including Ag(+), Hg(2+), and Cu(2+) showed strong inhibition on soybean urease, with the silver ion being a potent inhibitor (IC(50) = 2.3 x 10(-8) mM). Time-dependent inhibition studies exhibited biphasic kinetics with all heavy metal ions. Furthermore, inhibition studies with sodium salts of mineral acids (NaF, NaCl, NaNO(3), and Na(2)SO(4)) showed that only F(-) inhibited soybean urease significantly (IC(50) = 2.9 mM). Competitive type of inhibition was observed for this anion with a K(i) value of 1.30 mM.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP5467743	4-Bromophenylboronic acid		5467-74-3	Price

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