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Inhibitory Effect of the Gut Microbial Linoleic Acid Metabolites, 10-oxo-trans-11-octadecenoic Acid and 10-hydroxy-cis-12-octadecenoic Acid, on BV-2 Microglial Cell Activation

Shiori Ikeguchi, Yasuhiko Izumi, Nahoko Kitamura, Shigenobu Kishino, Jun Ogawa, Akinori Akaike, Toshiaki Kume

J Pharmacol Sci. 2018 Sep;138(1):9-15.

PMID: 30243517

Abstract:

10-oxo-trans-11-octadecenoic acid (KetoC) and 10-hydroxy-cis-12-octadecenoic acid (HYA) are long-chain fatty acids generated from linoleic acid by the gut lactic acid bacterium Lactobacillus plantarum. These fatty acids have been reported to have anti-inflammatory activity in the intestine. However, little is known about their effects in the brain. In this study, we aimed to investigate the effects of these fatty acids on lipopolysaccharide (LPS)-induced inflammatory processes in mouse microglial cells (BV-2 cells). KetoC and HYA inhibited LPS-induced nitric oxide (NO) production and suppressed the expression of inducible NO synthase in BV-2 cells. NO changes in these inhibitory effects were observed with AH7614, a G-protein coupled receptor 120 antagonist, or the peroxisome proliferator-activated receptors antagonists, GW6471 and GW9662. In addition, KetoC and HYA did not inhibit translocation of p65, a subunit of NF-κB, or IκB degradation. Similarly, no effect on p38 or JNK phosphorylation was observed. However, KetoC and HYA were found to inhibit ERK phosphorylation induced by LPS, suggesting that these fatty acids may exert their anti-inflammatory effects through the inhibition of ERK activation in microglial cells.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP880635030 GW6471 GW6471 880635-03-0 Price
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