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Inhibitory Effects of Costunolide Isolated From Laurus Nobilis on IgE-induced Degranulation of Mast Cell-Like RBL-2H3 Cells and the Growth of Y16 pro-B Cells

Inhibitory Effects of Costunolide Isolated From Laurus Nobilis on IgE-induced Degranulation of Mast Cell-Like RBL-2H3 Cells and the Growth of Y16 pro-B Cells

Tae Joon Kim, Kung-Woo Nam, Bora Kim, Sung-Jin Lee, Ki-Bong Oh, Kyeong Ho Kim, Woongchon Mar, Jongheon Shin

Phytother Res. 2011 Sep;25(9):1392-7.

PMID: 21674633

Abstract:

This study investigated the inhibitory effects of costunolide isolated from the leaves of Laurus nobilis L. (Lauraceae) on basophil-mediated allergic reactions and interleukin (IL)-5-mediated B cell growth. The effects of costunolide on β-hexosaminidase (a key parameter of degranulation) release and IL-4 expression in rat basophilic leukemia (RBL-2H3) cells were determined by measuring β-hexosaminidase activity and by semi-quantitative RT-PCR, respectively. The effects of costunolide on Y16 pro-B cell viability and growth were determined by 3-(4,5-dimethylthiazol-2-yl)2,5-diphenyltetrazolium bromide (MTT) assay. Costunolide was found significantly to inhibit β-hexosaminidase activity (p < 0.01) and IL-4 transcription in RBL-2H3 cells in a dose-dependent manner. Its 50% inhibitory concentration (IC50 ) was 34 µM, while that of the positive control, ketotifen, was 24 µM (IL-4 mRNA transcription). Moreover, costunolide dose-dependently suppressed pro-B cell growth in IL-5-stimulated Y16 cells. These results provide evidence that costunolide stabilizes mast cells by inhibiting IgE-mediated degranulation and inhibits IL-5-stimulated B cell growth.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP429653736	Y16		429653-73-6	Price

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