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Interaction of 5-hydroxy-l-tryptophan and Negative Dietary Cation-Anion Difference on Calcium Homeostasis in Multiparous Peripartum Dairy Cows

Interaction of 5-hydroxy-l-tryptophan and Negative Dietary Cation-Anion Difference on Calcium Homeostasis in Multiparous Peripartum Dairy Cows

C J Slater, E L Endres, S R Weaver, A A Cheng, M R Lauber, S F Endres, E Olstad, A DeBruin, P M Crump, E Block, L L Hernandez

J Dairy Sci. 2018 Jun;101(6):5486-5501.

PMID: 29605319

Abstract:

Hypocalcemia affects almost 50% of all dairy cows. Our laboratory has previously demonstrated that infusions of the serotonin precursor 5-hydroxy-l-tryptophan (5-HTP) increase circulating calcium concentrations in the Holstein transition cow. It is unknown whether feeding a negative dietary cation-anion difference (DCAD) diet alters the relationship between 5-HTP and hypocalcemia. The main objective of this study was to determine whether feeding a negative DCAD (-DCAD) diet before calving in conjunction with 5-HTP treatment could further diminish the magnitude of hypocalcemia at the time of calving. We used a randomized complete block design with a 2 × 2 factorial arrangement. Thirty-one multiparous Holstein cows were fed either a positive (+13 mEq/100 g) or negative (-13 mEq/100 g) DCAD diet 21 d before parturition and were intravenously infused daily with saline or 5-HTP (1 mg/kg) starting 7 d before the estimated date of parturition. Cows were blocked by parity and were randomly assigned to 1 of 4 treatment groups: positive DCAD plus saline, positive DCAD plus 5-HTP, negative DCAD plus saline, and negative DCAD plus 5-HTP, resulting in n = 8 per group. Total calcium (tCa), ionized calcium (iCa), and feed intake were recorded. The iCa was elevated prepartum in the -DCAD/5-HTP group compared with the other treatment groups as well as on d 0 and 1 postpartum. Although differences in tCa were not significant across the pre- or postpartum periods, tCa was numerically higher on d 0 and significantly higher on d 1 in -DCAD/5-HTP cows compared with all other groups. Prepartum the -DCAD/5-HTP treatment group ate less than the other treatment groups; however, postpartum dry matter intake differences were not significant. These findings demonstrate that feeding a -DCAD diet in conjunction with 5-HTP prepartum can increase postpartum circulating iCa concentrations and therefore diminish the magnitude of hypocalcemia at the time of parturition.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP4350098	5-Hydroxy-L-tryptophan		4350-09-8	Price

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