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Interaction of Cholera Toxin B Subunit With T and B Lymphocytes

Interaction of Cholera Toxin B Subunit With T and B Lymphocytes

Elena V Navolotskaya, Vladimir B Sadovnikov, Dmitry V Zinchenko, Valery M Lipkin, Vladimir P Zav'yalov

Int Immunopharmacol. 2017 Sep;50:279-282.

PMID: 28719851

Abstract:

We have prepared 125I-labeled cholera toxin B subunit (125I-labeled CT-B, a specific activity of 98Ci/mmol) and found that its binding to T and B lymphocytes from the blood of healthy donors was high-affinity (Kd 2.8 and 3.0nM, respectively). The binding of labeled protein was completely inhibited by unlabeled thymosin-α1 (TM-α1), interferon-α2 (IFN-α2), and the synthetic peptide LKEKK that corresponds to residues 16-20 in TM-α1 and 131-135 in IFN-α2, but was not inhibited by the synthetic peptide KKEKL with inverted amino acid sequence (Ki>10μM). Thus, TM-α1, IFN-α2, and the peptide: LKEKK bind with high affinity and specificity to CT-B receptor on donor blood T and B lymphocytes. It was found that CT-B and the peptide: LKEKK at concentrations of 10-1000nM increased in a dose-dependent manner the soluble guanylate cyclase activity in T and B lymphocytes.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42414606	Cholera Toxin B subunit			Price

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