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Interaction of Naja Naja Atra Cardiotoxin 3 With H-trisaccharide Modulates Its Hemolytic Activity and Membrane-Damaging Activity

Interaction of Naja Naja Atra Cardiotoxin 3 With H-trisaccharide Modulates Its Hemolytic Activity and Membrane-Damaging Activity

Pei-Hsiu Kao, Shinne-Ren Lin, Long-Sen Chang

Toxicon. 2010 Jun 15;55(7):1387-95.

PMID: 20193704

Abstract:

To address whether saccharide moieties of blood groups A, B and O antigens modulate hemolytic activity of Naja naja atra cardiotoxins (CTXs), the present study was carried out. Unlike other CTX isotoxins, hemolytic activity of CTX3 toward blood group O cholesterol-depleted red blood cells (RBCs) was notably lower than that of blood groups A and B cholesterol-depleted RBCs. Conversion of blood group B RBCs into blood group O RBCs by alpha-galactosidase treatment attenuated the susceptibility for hemolytic activity of CTX3, suggesting that H-antigen affected hemolytic potency of CTX3. Pre-incubation with H-trisaccharide reduced hemolytic activity and membrane-damaging activity of CTX3. Moreover, CTX3 showed a higher binding capability with H-trisaccharide than other CTXs did. CD spectra showed that the binding with H-trisaccharide induced changes in gross conformation of CTX3. Self-quenching studies revealed that oligomerization of CTX3 was affected in the presence of H-trisaccharide. Taken together, our data suggest that the binding of CTX3 with H-antigen alters its membrane-bound mode, thus reducing its hemolytic activity toward blood group O cholesterol-depleted RBCs.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42410783	H-Trisaccharide			Price

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