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Interactions of Collagen Molecules in the Presence of N-hydroxysuccinimide Activated Adipic Acid (NHS-AA) as a Crosslinking Agent

Interactions of Collagen Molecules in the Presence of N-hydroxysuccinimide Activated Adipic Acid (NHS-AA) as a Crosslinking Agent

Min Zhang, Kun Wu, Guoying Li

Int J Biol Macromol. 2011 Nov 1;49(4):847-54.

PMID: 21856329

Abstract:

The effect of crosslinking agent on pepsin-soluble bovine collagen solution was examined using N-hydroxysuccinimide activated adipic acid (NHS-AA) as a crosslinker. Electrophoretic patterns indicated that crosslinks formed when NHS-AA was added. A higher polarity level deduced from the changes in the fluorescence emission spectrum of pyrene in the crosslinked collagen solution indicated that the formation of well-ordered aggregates was suppressed. The random aggregation of collagens was also observed by atomic force microscopy (AFM). Furthermore, the association of collagens into fibrils was influenced by crosslinking. Self-assembly was suppressed at 37°C; however, as temperature was increased to 39°C, a small amount of NHS-AA leaded to an improvement in the ability of self-aggregation. Although more random structure was brought about by crosslinking, self-aggregation might still be promoted as temperature was increased, accompanying by the thermal stability improvement of fibrils.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP9007345-F	Bovine Collagen Solution		9007-34-5	Price

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