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Interactions of Oxidatively Modified Calf Skin Collagen With Platelets and Phagocytes

Interactions of Oxidatively Modified Calf Skin Collagen With Platelets and Phagocytes

Milan Ciz, Hana Cizova, Katerina Pejchalova, Viera Jancinova, Ivan Goshev, Boryana Mihaylova, Radomir Nosal, Antonin Lojek

Neuro Endocrinol Lett. 2009;30 Suppl 1:128-32.

PMID: 20027158

Abstract:

Objectives:
The effects of non-modified and oxidatively modified calf skin collagen type I on platelet aggregation and the oxidative burst of phagocytes were examined in the framework of a general hypothesis that collagen, platelets and phagocytes cooperate to modulate the oxidative burst of phagocytes and the extent of oxidative stress.
Materials and methods:
Calf skin collagen type I was subjected to oxidative modification by hydrogen peroxide or hydroxyl radical. Thermal denaturation of collagen was performed in a spectrophotometer equipped with a temperature gradient device. The aggregation of isolated human platelets obtained after differential centrifugation was measured using a dual-channel aggregometer. The production of reactive oxygen species by human whole blood phagocytes was evaluated by luminol-enhanced chemiluminescence.
Results:
Oxidative modification of collagen samples was characterized by a decrease in denaturation transition temperature. Oxidatively modified samples showed a modified SDS-PAGE pattern, evidencing a significant destruction of the collagen. All oxidatively modified collagen samples, independent of the oxidation treatment applied, lost their platelet-aggregating and phagocyte oxidative burst-inducing activity.
Conclusion:
The results suggest that reactive oxygen species were able to modify collagen. On the other hand, oxidatively modified collagen lost its activating properties towards platelets and phagocytes.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP9007345-D	Collagen from calf skin		9007-34-5	Price

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