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Interleukin 4 Moderately Affects Competence of Pluripotent Stem Cells for Myogenic Conversion

Interleukin 4 Moderately Affects Competence of Pluripotent Stem Cells for Myogenic Conversion

Barbara Świerczek-Lasek, Jacek Neska, Agata Kominek, Łukasz Tolak, Tomasz Czajkowski, Katarzyna Jańczyk-Ilach, Władysława Stremińska, Katarzyna Piwocka, Maria A Ciemerych, Karolina Archacka

Int J Mol Sci. 2019 Aug 13;20(16):3932.

PMID: 31412558

Abstract:

Pluripotent stem cells convert into skeletal muscle tissue during teratoma formation or chimeric animal development. Thus, they are characterized by naive myogenic potential. Numerous attempts have been made to develop protocols enabling efficient and safe conversion of pluripotent stem cells into functional myogenic cells in vitro. Despite significant progress in the field, generation of myogenic cells from pluripotent stem cells is still challenging-i.e., currently available methods require genetic modifications, animal-derived reagents, or are long lasting-and, therefore, should be further improved. In the current study, we investigated the influence of interleukin 4, a factor regulating inter alia migration and fusion of myogenic cells and necessary for proper skeletal muscle development and maintenance, on pluripotent stem cells. We assessed the impact of interleukin 4 on proliferation, selected gene expression, and ability to fuse in case of both undifferentiated and differentiating mouse embryonic stem cells. Our results revealed that interleukin 4 slightly improves fusion of pluripotent stem cells with myoblasts leading to the formation of hybrid myotubes. Moreover, it increases the level of early myogenic genes such as Mesogenin1, Pax3, and Pax7 in differentiating embryonic stem cells. Thus, interleukin 4 moderately enhances competence of mouse pluripotent stem cells for myogenic conversion.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42413401	Interleukin-4 from mouse			Price

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