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Interleukin-7 Contributes to Human pro-B-cell Development in a Mouse Stromal Cell-Dependent Culture System

Interleukin-7 Contributes to Human pro-B-cell Development in a Mouse Stromal Cell-Dependent Culture System

Tomoko Taguchi, Hisami Takenouchi, Yusuke Shiozawa, Jun Matsui, Noriko Kitamura, Yoshitaka Miyagawa, Yoko U Katagiri, Takao Takahashi, Hajime Okita, Junichiro Fujimoto, Nobutaka Kiyokawa

Exp Hematol. 2007 Sep;35(9):1398-407.

PMID: 17656007

Abstract:

Objective:
The role of interleukin (IL)-7 in human B lymphopoiesis is still controversial. We used an in vitro culture system to verify involvement of IL-7 in development of human pro-B cells from hematopoietic stem cells.
Materials and methods:
Human CD34(+) bone marrow cells were cultured for 4 weeks on MS-5 mouse stromal cells to induce pro-B cells. Expression of IL-7 receptor alpha or other B-cell differentiation marker genes on cultured human CD34(+)bone marrow cells was investigated by reverse transcription polymerase chain reaction (RT-PCR). Colony assay of human CD34(+) bone marrow cells was also performed to determine the effect of IL-7 on colony-forming ability. Neutralizing antibody or reagent that eliminates the effect of IL-7 was added to the culture system, and the number of pro-B cells induced was estimated by flow cytometry.
Results:
RT-PCR analysis revealed mRNA expression of IL-7 receptor alpha as well as B-cell differentiation marker genes in not only CD19(+) pro-B cells but also CD19(-) CD33(-) cells induced from CD34(+) bone marrow cells after cultivation for 4 weeks on MS-5 cells. Addition of anti-mouse IL-7 antibody, anti-human IL-7 receptor alpha antibody, or JAK3 kinase inhibitor reduced the number of pro-B cells induced, demonstrating that elimination of IL-7 reduces pro-B-cell development. Addition of anti-mouse IL-7 antibody emphasized the colony-forming ability of burst-forming unit erythroid cells.
Conclusions:
IL-7 produced by MS-5 cells is required for human pro-B-cell development from CD34(+)bone marrow cells in our culture system, and IL-7 appears to play a certain role in early human B lymphopoiesis.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR4248612	Interleukin-7 human			Price

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