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Intestinal Renal Metabolism of L-citrulline and L-arginine Following Enteral or Parenteral Infusion of L-alanyl-L-[2,15N]glutamine or L-[2,15N]glutamine in Mice

Intestinal Renal Metabolism of L-citrulline and L-arginine Following Enteral or Parenteral Infusion of L-alanyl-L-[2,15N]glutamine or L-[2,15N]glutamine in Mice

Petra G Boelens, Paul A M van Leeuwen, Cornelis H C Dejong, Nicolaas E P Deutz

Am J Physiol Gastrointest Liver Physiol. 2005 Oct;289(4):G679-85.

PMID: 15933220

Abstract:

Previously, we observed increased plasma arginine (ARG) concentrations after glutamine (GLN)-enriched diets, in combination with clinical benefits. GLN delivers nitrogen for ARG synthesis, and the present study was designed to quantify the interorgan relationship of exogenous L-GLN or GLN dipeptide, by enteral or parenteral route, contributing to intestinal citrulline (CIT) and renal de novo ARG synthesis in mice. To study this, we used a multicatheterized mouse model with Swiss mice (n = 43) in the postabsorptive state. Stable isotopes were infused into the jugular vein or into the duodenum {per group either free L-[2,(15)N]GLN or dipeptide L-ALA-L-[2,(15)N]GLN, all with L-[ureido-(13)C-(2)H(2)]CIT and L-[guanidino-(15)N(2)-(2)H(2)]ARG} to establish renal and intestinal ARG and CIT metabolism. Blood flow was measured using (14)C-para-aminohippuric acid. Net intestinal CIT release, renal uptake of CIT, and net renal ARG efflux was found, as assessed by arteriovenous flux measurements. Quantitatively, more de novo L-[2,(15)N]CIT was produced when free L-[2,(15)N]GLN was given than when L-ALA-L-[2,(15)N]GLN was given, whereas renal de novo L-[2,(15)N]ARG was similar in all groups. In conclusion, the intestinal-renal axis is hereby proven in mice in that L-[2,(15)N]GLN or dipeptide were both converted into de novo renal L-[2,(15)N]ARG; however, not all was derived from intestinal L-[2,(15)N]CIT production. In this model, the feeding route and form of GLN did not influence de novo renal ARG production derived from GLN.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP202406971	L-Cysteine-13C3,15N		202406-97-1	Price
AP202407269	L-Tyrosine-13C9,15N		202407-26-9	Price

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