Home
Resources
Publications
Intracellular Chloride Ion Concentration in Differentiating Neuronal Cell and Its Role in Growing Neurite

Intracellular Chloride Ion Concentration in Differentiating Neuronal Cell and Its Role in Growing Neurite

Ken-Ichi Nakajima, Yoshinori Marunaka

Biochem Biophys Res Commun. 2016 Oct 14;479(2):338-342.

PMID: 27641671

Abstract:

Chloride ion (Cl-) is one of the most abundant anions in our body. Increasing evidence suggests that Cl- plays fundamental roles in various cellular functions. We have previously reported that electroneutral cation-chloride cotransporters, such as Na+-K+-2Cl- cotransporter 1 (NKCC1) and K+-Cl- cotransporter 1 (KCC1), are involved in neurite outgrowth during neuronal differentiation. In the present study, we studied if there is correlation between intracellular Cl- concentrations ([Cl-]i) and the length of growing neurites. We measured [Cl-]i in the cell body and growing neurite tips using halide-sensitive fluorescent dye N-(ethoxycarbonylmethyl)-6-methoxyquinolinium bromide (MQAE), revealing that [Cl-]i in the tip of growing neurite was higher than that in cell body in a single cell. Importantly, there was a significant positive correlation between the length of growing neurite and [Cl-]i in neurite tip. Bumtanide (BMT), an inhibitor of NKCC1, significantly inhibited neurite outgrowth and decreased [Cl-]i in neurite tip. The results obtained in the present study and our previous studies together strongly suggest that high [Cl-]i in neurite tip region is crucial for efficient neurite outgrowth.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP162558523	1-(Ethoxycarbonylmethyl)-6-methoxyquinolinium bromide		162558-52-3	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: