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Introducing Transglycosylation Activity Into Human Salivary Alpha-Amylase (HSA)

Judit Remenyik, Chandran Ragunath, Narayanan Ramasubbu, Gyöngyi Gyémánt, András Lipták, Lili Kandra

Org Lett. 2003 Dec 11;5(25):4895-8.

PMID: 14653701

Abstract:

Synthesis of 4-nitrophenyl 1-thio-beta-D-maltoside, maltotrioside, and maltotetraoside in yields up to 60% has been achieved by a Tyr151Met (Y151M) mutant of human salivary alpha-amylase. Y151M is capable of transferring maltose and maltotriose residues from a maltotetraose donor onto different p-nitrophenyl glycosides. (1)H and (13)C NMR studies revealed that the mutated enzyme preserved the stereo- and regioselectivity. The glycosylation took place at position 4 of the glycosyl acceptor, forming the alpha(1-4)glycosidic bond, exclusively. [reaction: see text]

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP56846390 4-Nitrophenyl β-D-maltoside 4-Nitrophenyl β-D-maltoside 56846-39-0 Price
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