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Investigation of Selective Cytotoxicity and Determination of Ligand Induced Apoptosis of a New Acenaphtho [1,2-b] Quinoxaline Derivative

Investigation of Selective Cytotoxicity and Determination of Ligand Induced Apoptosis of a New Acenaphtho [1,2-b] Quinoxaline Derivative

Elham Mahjoob, Ali Khalaj, Seyed Nasser Ostad, Ebrahim Azizi, Shamileh Fouladdel, Shohreh Tavajohi, Raha Salehi, Reza Dowlatabadi

Arzneimittelforschung. 2009;59(10):526-31.

PMID: 19998581

Abstract:

Several new acenaphtho[1,2-b]quinoxaline derivatives were prepared by the reaction of o-phenylenediamines with acenaphthenequinones. The response of different carcinoid cell lines to these compounds were evaluated by MTT (3-(4,5-dimethylthiazol-2-yl)2,5-diphenyl tetrazolium bromide) assay and trypan blue exclusion tests. The cytotoxicity of 3,4-dinitroacenaphtho[1,2-b]quinoxaline (IIId) on the tested cell lines was confirmed by both tests. Furthermore, the MTT test showed a significant difference (p < 0.05) between the cytotoxicity of this compound on malignant cell lines of Caco-2, HT-29, T47D and non malignant mouse fibroblast cell line of NIH-3T3. An apoptosis inducing effect of compound IIId on K562 cells was detected by flow cytometry using Annexin-V-fluorescein isothiocyanate (AnV-FITC) and propidium iodide (PI) staining. The apoptosis induction (PI-/AnV+) in treated K562 cells was significantly (p < 0.01) more at 0.5 microg/ml concentration of compound IIId in comparison to all other concentrations of this compound and also doxorubicin (CAS 25316-40-9) (250 nM).

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP25316409-A	Doxorubicin, Hydrochloride - CAS 25316-40-9		25316-40-9	Price

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