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Investigation of the Chemical Profile and Cytotoxicity Evaluation of Organic Components Eluted From Pit and Fissure Sealants

Investigation of the Chemical Profile and Cytotoxicity Evaluation of Organic Components Eluted From Pit and Fissure Sealants

Elisabeth A Koulaouzidou, Konstantina Roussou, Konstantinos Sidiropoulos, Alexander Nikolaidis, Ioannis Kolokuris, Andreas Tsakalof, Christina Tsitsimpikou, Dimitrios Kouretas

Food Chem Toxicol. 2018 Oct;120:536-543.

PMID: 30076914

Abstract:

The aim of this study was to identify organic components eluted from five resin dental sealants using gas chromatography and mass spectrometry (GC/MS) after 1-day and 40-days storage and the effect of sealants on cell survival of cultured fibroblasts. Five resin materials were studied: BeautiSealant (SHOFU), Clinpro (3M/ESPE), Conseal F (SDI), Grandio Seal (VOCO) and Helioseal Clear (Ivoclar/Vivadent). The organic monomers detected were butylated hydroxytoluene (BHT), bis-phenol-A (BPA), camphoroquinone (CQ), diethylenglycoldimethacrylate (DEGDMA), 4N, N-dimethylaminobenzoic acid butylethoxyester (DMABEE), hydroxyethylmethcrylate (HEMA), hydroquinone monomethylether (MEHQ), triethylene glycol dimethacrylate (TEGDMA), tetrabutylammonium tetrafluoroborate (TBATFB), triphenylstibane (TPSb). The main monomer detected was TEGDMA, whereas BHT and DEGDMA were detected at lower concentrations. Higher monomer concentrations were detected after 40 days storage. The eluting chemical profiles of the tested materials differ qualitative and quantitative. For cytotoxicity evaluation, NIH/3T3 cells were exposed to eluates of sealants and cell viability was assessed by a quantitative technique at two observation periods. Decreased cell viability was observed. The cytotoxicity and the release of monomers from dental materials examined depends on the type of material and the observation time point. Resin-based dental materials have raised public concerns regarding possible adverse biological effects, thus it is essential to evaluate possible side effects for human health.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP429425	Tetrabutylammonium tetrafluoroborate		429-42-5	Price

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