Home
Resources
Publications
Investigation of the Complex Structure, Comparative DNA-binding and DNA Cleavage of Two Water-Soluble Mono-Nuclear lanthanum(III) Complexes and Cytotoxic Activity of Chitosan-Coated Magnetic Nanoparticles as Drug Delivery for the Complexes

Investigation of the Complex Structure, Comparative DNA-binding and DNA Cleavage of Two Water-Soluble Mono-Nuclear lanthanum(III) Complexes and Cytotoxic Activity of Chitosan-Coated Magnetic Nanoparticles as Drug Delivery for the Complexes

Zahra Asadi, Neda Nasrollahi, Hamidreza Karbalaei-Heidari, Vaclav Eigner, Michal Dusek, Nabiallah Mobaraki, Roya Pournejati

Spectrochim Acta A Mol Biomol Spectrosc. 2017 May 5;178:125-135.

PMID: 28178588

Abstract:

Two water-soluble mono-nuclear macrocyclic lanthanum(III) complexes of 2,6-diformyl-4-methylphenol with 1,3-diamino-2-propanol (C1) or 1,3-propylenediamine (C2) were synthesized and characterized by UV-Vis, FT-IR, 13C and 1H NMR spectroscopy and elemental analysis. C1 complex was structurally characterized by single-crystal X-ray diffraction, which revealed that the complex was mononuclear and ten-coordinated. The coordination sites around lanthanum(III) were occupied with a five-dentate ligand, two bidentate nitrates, and one water molecule. The interaction of complexes with DNA was studied in buffered aqueous solution at pH7.4. UV-Vis absorption spectroscopy, emission spectroscopy, circular dichroism (CD) and viscometric measurements provided clear evidence of the intercalation mechanism of binding. The obtained intrinsic binding constants (Kb) 9.3×103 and 1.2×103M-1 for C1 and C2, respectively confirmed that C1 is better intercalator than C2. The DNA docking studies suggested that the complexes bind with DNA in a groove binding mode with the binding affinity of C1>C2. Moreover, agarose gel electrophoresis study of the DNA-complex for both compounds revealed that the C1 intercalation cause ethidium bromide replacement in a competitive manner which confirms the suggested mechanism of binding. Finally, the anticancer experiments for the treated cancerous cell lines with both synthesized compounds show that these hydrophilic molecules need a suitable carrier to pass through the hydrophobic nature of cell membrane efficiently.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP13536793	Lanthanum(III) bromide		13536-79-3	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: