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[Investigation on the Interaction Between Pentadecafluorooctanoic Acid and Human Serum Albumin by Capillary Electrophoresis]

[Investigation on the Interaction Between Pentadecafluorooctanoic Acid and Human Serum Albumin by Capillary Electrophoresis]

Yi Gu, Ming Guo, Da Lü, Ping Hou, Xinxin Yin

Se Pu. 2018 Jan 8;36(1):69-77.

PMID: 29582615

Abstract:

Capillary electrophoresis (CE) has been used to establish the analytical method of interaction between pentadecafluorooctanoic acid (PFOA) and human serum albumin (HSA). Under the physiological conditions, the interaction model of PFOA and HSA were constructed. Mobility method, plug-plug kinetic (PPK) method and simplified Hummel-Dreyer method were used to determine the interaction between derivatives and HSA. Non-linear regression, Scatchard equation and Klotz equation were adopted to obtain the interaction parameters. The results showed that all the three methods can be used to analyze the interaction of PFOA-HSA system. According to the interaction parameters, the most suitable CE method is simplified Hummel-Dreyer method while the most suitable theoretical equation is non-linear regression. The binding parameters indicated that the interaction of PFOA-HSA system has only one type of binding sites and the binding is stable. The research results have illustrated the interaction between HSA and PFOA, and provided a beneficial reference for in-depth research of the toxic mechanism of PFOA.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP335671-A	Pentadecafluorooctanoic acid		335-67-1	Price

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