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Involvement of Beta 1,4 Galactosyltransferase 1 and Gal beta1-->4GlcNAc Groups in Human Hepatocarcinoma Cell Apoptosis

Involvement of Beta 1,4 Galactosyltransferase 1 and Gal beta1-->4GlcNAc Groups in Human Hepatocarcinoma Cell Apoptosis

Si Zhang, Mingmei Cai, Song Wen Zhang, Yun Hu, Jian X Gu

Mol Cell Biochem. 2003 Jan;243(1-2):81-6.

PMID: 12619892

Abstract:

Beta 1,4 galactosyltransferase 1 (beta 1,4GT1) synthesizes Gal beta 1-->4GlcNAc groups in N-linked sugar chains of animal glycoproteins, which have been demonstrated to play an important role in many biological events, including sperm-egg interaction, cell migration and mammalian embryonic development. In this study, the mRNA level of beta 1,4GT1 was found to increase greatly during the 7721 hepatocarcinoma cells apoptosis induced by cycloheximide. Ricinus Communis Agglutinin-I staining indicated generous increase of Gal beta 1-->4GlcNAc groups during apoptosis. Further study showed that the 7721 hepatocarcinoma cells transiently transfected with beta 1,4GT1 were more susceptible to the apoptosis induced by cycloheximide. The increased susceptibility was in accordance to the transfection concentration of beta 1,4GT1, which also led to the increased Gal beta 1-->4GlcNAc groups on the transfected cell surface. All the observations suggested that beta 1,4GT1 and Gal beta 1-->4GlcNAc groups might be associated with the apoptosis of human hepatocarcinoma cells.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42410733	Beta-1,4-galactosyltransferase 1			Price

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